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PO0013 Enzyme Activities (Fishman units) Correlate Poorly with Hydrolysis E?iciencies
來源:IMCS | 作者:Cathleen Melendez*, Margarita Marinova, P. Nikki Sitasuwan, L. Andrew Lee | 發布時間: 71天前 | 38 次瀏覽 | 分享到:
?Activity of ?ve di?erentβ-glucuronidases was tested via a phenolphthalein-glucuronide chromogenic assay.
?Hydrolysis e?ciency of ten drugs was monitored for all ?veβ-glucuronidases at ?vetime points.
?Activity level measured with phenolphthalein-glucuronide did not correlate well withdrug hydrolysis e?ciency.

β-glucuronidase is an enzyme commonly utilized in clinical and forensic labs toconvert drug metabolites present in biological ?uids into their parent compoundsfor better detection and quanti?cation. Since Fishman et al. introduced a quickand simple way to determineβ-glucuronidase activity in the 1940s, the



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