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無錫微色譜生物科技有限公司
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JA0051 The Analysis of ?9-Tetrahydrocannabinol and Metabolite in Whole Blood and 11-Nor-?9- Tetrahydrocannabinol-9-Carboxylic Acid in Urine Using Disposable Pipette Extraction with Confirmation and Quantification by Gas Chromatography– Mass Spectrometry
來源:Journal of Analytical Toxicology | 作者:Jennifer L. Schroeder | 發布時間: 36天前 | 93 次瀏覽 | 分享到:
Essential to forensic laboratories is the desire to find a more sensitive, rapid method of analyzing ?9-tetrahydrocannabinol (THC) and metabolite in biological specimens. Disposable pipette extraction (DPX) is a valuable method in extracting THC and 11- nor-?9-tetrahydrocannabinol-9-carboxylic acid (THCc) in blood and THCc in urine. Less waste and solvent usage; smaller specimen volume; clean chromatograms; and utilization of lowcost equipment and consumables were achieved using this method. Differing from traditional solid-phase extraction devices, DPX uses loosely packed sorbent allowing thorough mixing with the specimen without requiring vacuum for elution. Prior to extraction, urine specimens were hydrolyzed and proteins precipitated from blood. Specimen volume requirements were 1 mL of blood and 0.2 mL of urine. The limits of quantitation for THC and THCc in blood were 1 and 2 ng/mL, respectively, and 3 ng/mL for THCc in urine. With R2 values ≥?0.99, blood calibration curves were linear from 1 to 200 ng/mL and 2 to 500 ng/mL for THC and THCc, respectively, with urine THCc linear from 3 to 2000 ng/mL.
1 Introduction

Marijuana is a commonly abused drug because of its central nervous system depressant effects and its hallucinogenic properties. The primary pharmacologically active component in marijuana is ?9-tetrahydrocannabinol (THC). The pathway for metabolism and excretion of THC leads primarily to the formation of 11-hydroxy-?9-tetrahydrocannabinol (THCoh), which further metabolizes to 11-nor-? 9-tetrahydrocannabinol-9-carboxylic acid (THCc) (1). Unchanged THC exists in urine in only trace amounts, and THCoh accounts for only 2% of the excreted dose (2). The detection of THCc and its conjugates, which are the major urinary metabolites of THC, is also important in determining exposure to marijuana. The detection of THC in blood is important in human performance and postmortem toxicology in order to correlate recent drug use with impaired performance or behavior. Detection and quantification of low blood concentrations of THC are required because of THC’s short half life and the time gap that occurs between the collection of the specimen and the human performance event. Because of marijuana’s popularity and frequency of abuse, forensic toxicology laboratories must have a rapid, sensitive, and inexpensive method for determining the presence of major marijuana constituents in biological specimens. In addition, Ohio Department of Health (ODH) regulations require all laboratories in Ohio to report concentrations of THC and THCc in blood specimens down to 2 and 5 ng/mL, respectively, and 15 ng/mL THCc in urine. Methods consisting of liquid–liquid and solid-phase extraction (SPE) have been published (3–20). These methods focus primarily on the separation of acidic and basic fractions, resulting in two separate chromatographic analyses for THC and THCc.


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